Promoted migration, invasion and metastasis, and reduced the sensitivity of NSCLC cells to docetaxel. Results: Overexpression of TPX2 was related to the poor prognosis of NSCLC. Gene expression was detected via Western blot and real time PCR (RT-qPCR). Exosomes were extracted by differential centrifugation from the culture supernatant, and their functions were investigated by co-culture with tumor cells. An in vivo lung homing mouse model was used to further confirmed the role of TPX2 on metastasis. Stable TPX2 overexpression cell lines with were constructed by lentivirus infection, and the effect of TPX2 on proliferation, migration, invasion and chemoresistance to docetaxel was characterized by the CCK8, wound healing, transwell, colony formation assay and FACS. Methods: Bioinformatics analysis was used to analyze the relationship between TPX2 and the clinicopathological features of NSCLC. However, the role and mechanism of Targeting protein for Xenopus kinesin-like protein 2 (TPX2), which is highly expressed in NSCLC, is still unclear. Jiaru Hu, 1 Qing He, 1 Tian Tian, 2 Na Chang, 3 Liting Qian 1ġDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, People’s Republic of China 2Department of Respiratory Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Anhui Provincial Cancer Hospital, Hefei, 230001, People’s Republic of China 3Department of Radiation Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Anhui Provincial Cancer Hospital, Hefei, 230001, People’s Republic of ChinaĬorrespondence: Liting Qian, Email īackground: Lung cancer, most of which is non-small cell lung cancer (NSCLC), is the most common tumor in the world, and drug resistance, as a major problem in clinical treatment, has attracted extensive attention.
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